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KMID : 0545120150250101648
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Journal of Microbiology and Biotechnology
2015 Volume.25 No. 10 p.1648 ~ p.1652
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A New Protein Factor in the Product Formation of Non-Reducing Fungal Polyketide Synthase with a C-Terminus Reductive Domain
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Balakrishnan Bijinu
Chandran Ramya
Park Si-Hyung
Kwon Hyung-Jin
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Abstract
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Azaphilone polyketides are synthesized by iterative non-reducing fungal polyketide synthases (NR-fPKSs) with a C-terminus reductive domain (-R). Several azaphilone biosynthetic gene clusters contain a putative serine hydrolase gene; the Monascus azaphilone pigment (MAzP) gene cluster harbors mppD. The MAzP productivity was significantly reduced by a knockout of mppD, and the MAzP NR-fPKS-R gene (MpPKS5) generated its product in yeast only when co-expressed with mppD. Site-directed mutations of mppD for conserved Ser/Asp/His residues abolished the product formation from the MpPKS5/mppD co-expression. MppD and its homologs are thus proposed as a new protein factor involved in the product formation of NR-fPKS-R.
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KEYWORD
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Monascus pigment, azaphilone polyketide, non-reducing fungal polyketide synthase, MppD, Ser/Asp/His catalytic triad
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